Rose was born to Katia Langenheim, a 20-year-old single mother and artist, who she now describes as one of her role-models. As a young child, she travelled frequently, living in rural Victoria, Tasmania and Surfers Paradise before finally settling in Melbourne. As a teenager, she attended University High School. Ruby is the god-daughter of Lionel Rose and the great-granddaughter of Alec Campbell, the last surviving Battle of Gallipoli soldier.
In order to land her gig as an MTV VJ in Australia, she competed against 2000 other hopefuls in a three-week national search, as former VJ Lyndsey Rodrigues moved to the United States to co-host TRL. As part of the competition, she downed 100 shots of beer in 100 minutes against Jackass' Bam Margera, and kissed strangers on a busy Sydney street. Rose has stated that she wanted to be a musician rather than an MTV VJ, however she cannot play any instruments. However, she enjoys her job as VJ compared to her previous job as a model. "Being a model there is always something they want to change. Whether they want someone a little bit skinnier, a little bit taller, a little bit prettier, but MTV want you to be yourself ... not censoring anything and not conforming to anything". Ironically, around the same time Rose won the search, MTV Australia axed most of its music-based programming, so Rose's actual on-air time has been reduced to short filler segments between shows such as The Hills and Pimp My Ride.
In 2009, Rose won the ASTRA Award for Favorite Female Personality. She also travelled to Kenya to "highlight ... [the] amazing work" done by Global Vision International. She appeared on the first episode of Talkin' 'Bout Your Generation, representing Generation Y alongside comedian Josh Thomas.
She appeared in the episode "Media Virgins", of Australia's Next Top Model, acting as a guest judge. She also worked as a correspondent for the finale of Australia's Next Top Model.
From July 2009, Rose along with Dave Hughes, Charlie Pickering, Carrie Bickmore and James Mathison host The 7pm Project, an Australian television news talk show produced by Roving Enterprises which airs weeknights on Network Ten.